Microarray:Article Title: Activity of the Bacillus anthracis 20 kDa protective antigen component
Article Snippet: RNA quantity was determined using a Nanodrop spectrophotometer. .. Preparation of Microarray Chip: Human cDNA microarrays were generated using sequence verified PCR elements including the approximate 6900 well-characterized human genes from The Easy to Spot Human UniGEM V2.0 cDNA (Incyte Genomics, Inc). .. The PCR products ranging from 500–700 base pairs were deposited in 3X saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Generated:Article Title: Activity of the Bacillus anthracis 20 kDa protective antigen component
Article Snippet: RNA quantity was determined using a Nanodrop spectrophotometer. .. Preparation of Microarray Chip: Human cDNA microarrays were generated using sequence verified PCR elements including the approximate 6900 well-characterized human genes from The Easy to Spot Human UniGEM V2.0 cDNA (Incyte Genomics, Inc). .. The PCR products ranging from 500–700 base pairs were deposited in 3X saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Sequencing:Article Title: Activity of the Bacillus anthracis 20 kDa protective antigen component
Article Snippet: RNA quantity was determined using a Nanodrop spectrophotometer. .. Preparation of Microarray Chip: Human cDNA microarrays were generated using sequence verified PCR elements including the approximate 6900 well-characterized human genes from The Easy to Spot Human UniGEM V2.0 cDNA (Incyte Genomics, Inc). .. The PCR products ranging from 500–700 base pairs were deposited in 3X saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Article Title: Biomarkers of immune dysfunction in response to chronic stress, methods of use and diagnostic kits
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, Calif.) optical scanner, and the data were documented using Gene Pix 6.0 (Axon Instruments Inc, Union City, Calif.). .. Preparation of cDNA Microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from ̃10,000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, Del.). .. The PCR products, ranging from 500 to 700 base pairs, were deposited in 3× saline sodium citrate (SSC) at an average concentration of 165 μg/ml on CMT-GAPSTM II (γ-aminopropylsilane) coated slides (Corning Inc., Corning, N.Y.), using a Bio-Rad VersArray MicroArrayer (Hercules, Calif.).
Article Title: Transcriptome characterization of immune suppression from battlefield-like stress.
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, CA, USA) optical scanner, and the images were digitized using GenePix 6.0 software (Axon Instruments Inc.). .. Preparation of cDNA microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from B10 000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, DE, USA). .. The PCR products, ranging between 500 and 700 bp, were deposited in 3 saline sodium citrate at an average concentration of 165mg ml 1 on CMT-GAPS II (g-aminopropylsilane) coated slides (Corning Inc., Corning, NY, USA), using VersArray MicroArrayer (Bio-Rad, Hercules, CA, USA).
Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Polymerase Chain Reaction:Article Title: Activity of the Bacillus anthracis 20 kDa protective antigen component
Article Snippet: RNA quantity was determined using a Nanodrop spectrophotometer. .. Preparation of Microarray Chip: Human cDNA microarrays were generated using sequence verified PCR elements including the approximate 6900 well-characterized human genes from The Easy to Spot Human UniGEM V2.0 cDNA (Incyte Genomics, Inc). .. The PCR products ranging from 500–700 base pairs were deposited in 3X saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Article Title: Biomarkers of immune dysfunction in response to chronic stress, methods of use and diagnostic kits
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, Calif.) optical scanner, and the data were documented using Gene Pix 6.0 (Axon Instruments Inc, Union City, Calif.). .. Preparation of cDNA Microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from ̃10,000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, Del.). .. The PCR products, ranging from 500 to 700 base pairs, were deposited in 3× saline sodium citrate (SSC) at an average concentration of 165 μg/ml on CMT-GAPSTM II (γ-aminopropylsilane) coated slides (Corning Inc., Corning, N.Y.), using a Bio-Rad VersArray MicroArrayer (Hercules, Calif.).
Article Title: Transcriptome characterization of immune suppression from battlefield-like stress.
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, CA, USA) optical scanner, and the images were digitized using GenePix 6.0 software (Axon Instruments Inc.). .. Preparation of cDNA microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from B10 000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, DE, USA). .. The PCR products, ranging between 500 and 700 bp, were deposited in 3 saline sodium citrate at an average concentration of 165mg ml 1 on CMT-GAPS II (g-aminopropylsilane) coated slides (Corning Inc., Corning, NY, USA), using VersArray MicroArrayer (Bio-Rad, Hercules, CA, USA).
Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Produced:Article Title: Biomarkers of immune dysfunction in response to chronic stress, methods of use and diagnostic kits
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, Calif.) optical scanner, and the data were documented using Gene Pix 6.0 (Axon Instruments Inc, Union City, Calif.). .. Preparation of cDNA Microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from ̃10,000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, Del.). .. The PCR products, ranging from 500 to 700 base pairs, were deposited in 3× saline sodium citrate (SSC) at an average concentration of 165 μg/ml on CMT-GAPSTM II (γ-aminopropylsilane) coated slides (Corning Inc., Corning, N.Y.), using a Bio-Rad VersArray MicroArrayer (Hercules, Calif.).
Article Title: Transcriptome characterization of immune suppression from battlefield-like stress.
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, CA, USA) optical scanner, and the images were digitized using GenePix 6.0 software (Axon Instruments Inc.). .. Preparation of cDNA microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from B10 000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, DE, USA). .. The PCR products, ranging between 500 and 700 bp, were deposited in 3 saline sodium citrate at an average concentration of 165mg ml 1 on CMT-GAPS II (g-aminopropylsilane) coated slides (Corning Inc., Corning, NY, USA), using VersArray MicroArrayer (Bio-Rad, Hercules, CA, USA).
Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
cDNA Library Assay:Article Title: Biomarkers of immune dysfunction in response to chronic stress, methods of use and diagnostic kits
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, Calif.) optical scanner, and the data were documented using Gene Pix 6.0 (Axon Instruments Inc, Union City, Calif.). .. Preparation of cDNA Microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from ̃10,000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, Del.). .. The PCR products, ranging from 500 to 700 base pairs, were deposited in 3× saline sodium citrate (SSC) at an average concentration of 165 μg/ml on CMT-GAPSTM II (γ-aminopropylsilane) coated slides (Corning Inc., Corning, N.Y.), using a Bio-Rad VersArray MicroArrayer (Hercules, Calif.).
Article Title: Transcriptome characterization of immune suppression from battlefield-like stress.
Article Snippet: Hybridized slides were scanned and recorded using a GenePix Pro 4000B (Axon Instruments Inc., Union City, CA, USA) optical scanner, and the images were digitized using GenePix 6.0 software (Axon Instruments Inc.). .. Preparation of cDNA microarrays Human cDNA microarrays were prepared using sequence-verified PCR elements produced from B10 000 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA Library (Incyte Genomics Inc., Wilmington, DE, USA). .. The PCR products, ranging between 500 and 700 bp, were deposited in 3 saline sodium citrate at an average concentration of 165mg ml 1 on CMT-GAPS II (g-aminopropylsilane) coated slides (Corning Inc., Corning, NY, USA), using VersArray MicroArrayer (Bio-Rad, Hercules, CA, USA).
Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Spectrophotometry:Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
Hybridization:Article Title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis)
Article Snippet: Total RNA was subsequently isolated from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA) following manufacturer protocol. .. RNA quantity was measured via spectrophotometry followed by analysis with a Bioanalyzer 2100 (Agilent Technologies, CA) Custom made cDNA Microarray Slide Preparation and Hybridization Human cDNA microarrays were prepared by using sequence verified PCR elements produced from approximately 6900 well-characterized human genes of The Easy to Spot Human UniGEM V2.0 cDNA library (Incyte Genomics, Inc). .. The PCR products, ranging from 500 to 700 bps, were deposited in 3·saline sodium citrate (SSC) at an average concentration of 165 μg/μl on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc).
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